| Gene name: | CLU |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Lung |
| Cell name: | -- |
| Gene ID: | 1191 |
| Category: | protein coding |
| Phenotype: | Idiopathic pulmonary fibrosis |
| Experimental category: | HL |
| PMID: | 29133960 |
| Experiment: | Knockdown//Histological staining//Western blot |
| Description: | After 14-days of bleomycin administration, both bleomycin-treated wildtype and CLU?/? mice had increased percent p-H2A.X+ cell clusters, as defined by clusters of 3 or more positive cells, but there were no significant differences between the genotypes. Further, there was no difference in either 8-Oxo-dG intensity or percent p-H2A.X+ cell clusters, 28-days after saline and bleomycin-treatment of wildtype mice. However, there was a significant increase in 8-Oxo-dG staining intensity and p-H2A.X+ cell clusters, in CLU?/? compared to wildtype mice, 28-days after bleomycin-challenge. Finally, exogenously administered Clusterin protein to saline or bleomycin treated wildtype mice did not modulate the number of p-H2A.X+ cell clusters or the percentage of 8-Oxo-dG positive cells in the airways. there was a significant decrease in the expression of msh2, msh6, and ogg1 transcripts in CLU?/? bleomycin-challenged mice compared to CLU?/? saline-control mice. Moreover, there was a significant decrease in msh6 in CLU?/? compared to wildtype mice following bleomycin challenge at Day 28. There was an increase in whole lung p21 and IL1? proteins and KC transcript expression in the bleomycin-challenged CLU?/? compared with their wildtype counterparts at this time point. Finally, there was a significant increase in sftpc transcript expression in wildtype but not CLU?/? mice, 28 days after bleomycin administration. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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