| Gene name: | PRMT5 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Brain |
| Cell name: | GBMNS |
| Gene ID: | 10419 |
| Category: | protein coding |
| Phenotype: | Glioblastoma |
| Experimental category: | HL |
| PMID: | 27292259 |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Knockdown |
| Description: | PRMT5 depletion increased the G1 population of GBMNS by atleast 30% implicating G1/S cell cycle arrest. Although control cells had undetectable β-gal-positive cells, there were more than 100 β-gal-positive cells per view field in the PRMT5-depleted GBMNS. |
| Target gene: | P53//P21//RB |
| Official symbol(s): | P53//P21//RB |
| R-AG-Target gene: | --//--// -- |
| Subcategory: | Unclear |
| Target gene experiment: | RT-PCR//Western blot//Knockdown |
| Target gene description: | PRMT5 knockdown increased the relative mRNA expression of p21 and p53. Further we also probed for the expression of Rb, p53 and p21 proteins with or without PRMT5 knockdown .Consistent with senescence phenotype, we found decreased pRb and increased expression of p53 and its target gene p21. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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