| Gene name: | SRC |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HT-1080 |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | Surprisingly, the fraction of SA-β-gal–positive cells was greatly reduced in the Src-expressing population, As expected, most of the control cells were arrested in the fraction with 4N DNA content.By contrast, BrdUrd incorporation was seen in the Src population with DNA content between 4N and 8N, suggesting that these cells exit mitosis and then re-replicate their DNA. |
| Target gene: | P21 |
| Official symbol(s): | P21 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | RT-PCR//Western blot |
| Target gene description: | As expected, RT-PCR analysis indicated that p21waf1 accumulated in control cells 36 to 72 hours after drug treatment. Surprisingly, p21waf1 was not significantly expressed in Src-expressing cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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