| Gene name: | EP400 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | U2OS,IMR-90 |
| Experiment: | SA-β-gal activity assay//Western blot//Flow cytometry//qPCR//DAPI staining//Knockdown |
| Description: | We found that p400 knockdown induces the activation of the gene encoding the p21 cell cycle-dependent kinase inhibitor, as well as the concomitant accumulation of cells in the G1 phase of the cell cycle. As already demonstrated [11], transfection of p400 siRNA leads to senescence of IMR90 cells, as observed by the appearance of the so-called SAHF (Senescence-Associated Heterochromatin Foci), the induction of Senescence–Associated β-Galactosidase activity and induction of p16 mRNA expression. |
| Target gene: | ATM |
| Official symbol(s): | ATM |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Immunofluorescence//Knockdown//Flow cytometry |
| Target gene description: | We observed by immunofluorescence staining that p400 knockdown induces ATM phosphorylation both in IMR90 cells and HCT116 cells.We found that the knockdown of p400 leads to an increase of ROS levels (measured by calculating the mean fluorescence from 25,000 cells) detectable 48 hours and 72 hours following siRNA transfection. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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