| Gene name: | RING1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HepG2,HCT116 |
| Gene ID: | 6015 |
| Category: | protein coding |
| Phenotype: | Hepatocellular carcinoma |
| Experimental category: | HL |
| PMID: | 29187402 |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | Significant induction of senescence was observed, as 3- to 7-fold more cells were positively stained in the RING1-deficient group showed than in the controls. FACS analysis also indicated that, upon RING1 depletion, increased cell subpopulations were found to retain in G1 phase (increased from 60.0% to 68.4% in HepG2 cells, and from 53.1% to 58.9% in HCT116 cells), suggesting that RING1 deficiency might at least cause partial G1 arrest. |
| Target gene: | P53 |
| Official symbol(s): | P53 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Knockdown//Western blot |
| Target gene description: | RING1 knockdown decreased the ubiquitination of p53.In a reconstituted in vitro ubiquitination assay that included E1, UbcH6 (E2), ATP and Ub, RING1 was also found to ubiquitinate p53. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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