| Gene name: | DDX24 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | U2OS |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Knockdown//Western blot//Cell morphological analysis |
| Description: | 43% of the total control cells arrested at G1 phase, and DDX24 knockdown led to the significant increase of G1 population to 62%. In accordance, the reduction of S phase is drastic when DDX24 is depleted from cells.DDX24 siRNA treated cells, under same treatment, did not undergo apoptosis but showed even more predominant G1/S arrest.prolonged culturing of DDX24 siRNA treated U2OS cells ultimately resulted in enlarged and flattened cell shape, the phenotypes typical of senescence45. DDX24 knockdown cells appeared nearly 100% positive for β-galactosidase staining, which proves the occurrence of senescence in cells lacking DDX24 protein. |
| Target gene: | P53 |
| Official symbol(s): | P53 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Knockdown//Western blot |
| Target gene description: | RNAi mediated knockdown of DDX24 in U2OS cells significantly augmented expression level of p21 and PUMA, the classic p53 targets in regulating cell cycle arrest and apoptosis. Therefore, DDX24 acts as a negative modulator of p53 transcriptional activity. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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