| Gene name: | DEK |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Melanocyte,SK-Mel-28,SK-Mel-94,G-361,UACC-62,UACC-257,MM576 |
| Experiment: | SA-β-gal activity assay//Flow cytometry//Cell morphological analysis |
| Description: | In 6 of 12 lines analyzed (SK-Mel-28, SK-Mel-94, G-361, UACC-62, UACC-257, and MM576), DEK depletion led to a progressive cell cycle arrest, eventually resulting in a near complete inhibition of cell proliferation as determined by total cell counting and flow cytometry.The reduction of cells in S phase of the cell cycle was accompanied by cell enlargement, flattening, and vacuolization reminiscent of classic senescent phenotypes . In fact, staining for senescence-associated β-galactosidase activity was positive in DEK-depleted melanoma cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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