| Gene name: | POT1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | BGC-823 |
| Gene ID: | 25913 |
| Category: | protein coding |
| Phenotype: | Gastric cancer |
| Experimental category: | HL |
| PMID: | 20517159 |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//qPCR |
| Description: | After 24 h of culturing, the cells were incubated with β-galactosidase and observed microscopically. In the control cells, only one or two cells showed weak staining. However, after silencing hPOT1 with RNAi, the cells appeared large and flat, some of which were strongly stained with blue. The ratio of senescent cells in blue was 144 ± 12.4/105 (n = 3).The telomere length assay showed that the T/S value = [2Ct (telomere)/2Ct (36B4)] – 1 of the hPOT1 RNAi cells (1.383 ± 0.091) was statistically less than that of the control cells (3.043 ±0.548) (P= 0.007, n= 3), indicating that the loss of hPOT1 in BGC823 cells leads to the shortening of the telomere. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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