| Gene name: | TGFB2 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HuH-7 |
| Gene ID: | 7042 |
| Category: | protein coding |
| Phenotype: | Hepatocellular carcinoma |
| Experimental category: | HL |
| PMID: | 21847365 |
| Experiment: | SA-β-gal activity assay//Flow cytometry |
| Description: | We also observed that addition of rhTGF-β2 induced cellular senescence in both Huh7 and HepG2 cells as shown by enhanced accumulation of SA-β-Gal. Huh7 and HepG2 cells were grown in culture medium supplemented with rhTGF-β2 (1 or 5 ng/ml) for a period of 48 hours before flow cytometry analyses. Similar to the effects of GPC3 suppression, significant increases in the G1 peak , together with a decrease in S- and G2-phase cells were observed in both cell lines compared with control cells not treated with rhTGF-β2 . |
| Target gene: | CYCLIN A//CYCLIN D1//RB//P15INK4B//P21//BCL-XL//MCL-1//BCL-2 |
| Official symbol(s): | CYCLIN A//CYCLIN D1//RB//P15INK4B//P21//BCL-XL//MCL-1//BCL-2 |
| R-AG-Target gene: | Downregulation//Downregulation//Downregulation//Upregulation//Upregulation//Downregulation//Downregulation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | Western blot analysis further showed that rhTGF-β2 decreased the expression of cell cycle regulators cyclin A and cyclin D1, as observed with GPC3 suppression . In addition, phosphorylation of retinoblastoma (Rb) protein was decreased, whereas the expression of p15Ink4b and p21Cip1 were increased in both Huh7 and HepG2 cells, indicating a G1 cell cycle arrest. The addition of TGF-β2 also increased the apoptotic response, demonstrated by the reduced expression of antiapoptotic proteins (Bcl-xL and Mcl-1) in both cell lines, and the reduced expression of Bcl-2 in HepG2 cells only (Huh7 cells do not express Bcl-2). |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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