| Gene name: | CDK2AP1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HDF |
| Experiment: | SA-β-gal activity assay//Knockdown |
| Description: | We found that 91.7% of HDFs transduced with CDK2AP1-shRNA1 and 70.8% of CDK2AP1-shRNA2 transduced cells displayed appearance of enlarged blue cells in contrast to the control EV transduced cells, which failed to exhibit a detectable blue appearance. |
| Target gene: | P53//P21//BAX//PUMA |
| Official symbol(s): | P53//P21//BAX//BBC3 |
| R-AG-Target gene: | --//--//--//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//qPCR |
| Target gene description: | Indeed we have found that HDFs in which CDK2AP1 was down regulated had a significant increase in p53 protein levels. we found that p21 mRNA levels increased by 2.1 fold in the CDK2AP1-shRNA1 cells and by 1.8 fold in the CDK2AP1-shRNA2 HDFs when compared with EV transduced cells. Western blot analysis further confirmed the increase in p21 protein levels.QPCR analysis indicated that BAX mRNA levels were increased by 1.5 fold in the CDK2AP1-shRNA1 transduced HDFs and by 2.4 fold in the CDK2AP1-shRNA2 when compared with EV transduced cells. Similarly, we observed that PUMA mRNA levels were increased by 2.0 fold in the CDK2AP1-shRNA1 cells and by 3.6 fold in cells transduced with CDK2AP1-shRNA2 when compared with EV cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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