| Gene name: | KDM4B |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HCT116,SW480 |
| Gene ID: | 23030 |
| Category: | protein coding |
| Phenotype: | Colorectal cancer |
| Experimental category: | HL |
| PMID: | 24473398 |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//Flow cytometry |
| Description: | Positive SA-β-gal staining was observed at 72 h post-transfection in about 49% and 20% of JMJD2B-silenced HCT116 and SW480 cells, respectively, but only <2% of the control group were SA-β-gal positive.Compared with the control group, there was a significant increase of HCT116 cells with maximum 4N content at 12?h, and an increase of SW480 cells with maximum 2N content at 24?h after JMJD2B silencing in hypoxia, indicative of G2 and G1 phase arrest, respectively. Morphologically, JMJD2B-silenced cells were larger and appeared flattened (data not shown), which is a feature of senescence (Itahana et al, 2007). |
| Regulatory pathway: | STAT3 |
| R-AG-Pathway: | -- |
| Official symbol(s): | STAT3 |
| Pathway experiment: | Western blot |
| Pathway description: | Although a slight decrease of total STAT3 was visually observed, p-STAT3 expression significantly decreased in JMJD2B-silenced cells in both normoxia and hypoxia, which was confirmed upon quantification. |
Annotation:
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