| Gene name: | TWIST1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | H460,A549,H358,H727,Calu-1,Calu-6,H1975 |
| Gene ID: | 7291 |
| Category: | protein coding |
| Phenotype: | Lung cancer |
| Experimental category: | HL |
| PMID: | 23364532 |
| Experiment: | SA-β-gal activity assay//Western blot//Knockdown//Cell morphological analysis//Flow cytometry |
| Description: | Representative photomicrographs (20×) of increased SA-β-gal staining in 3 representative NSCLC cell lines following shRNA mediated TWIST1 knockdown(above).We observed a dramatic activation of latent OIS as demonstrated by positive SA-β-Gal staining and characteristic enlarged, flattened cytoplasm morphology in three KRAS mutant NSCLC cells lines (H460, A549, and H358). These findings were further supported by profound cell cycle arrest in these cell lines. as well as by induction of the senescence markers, p21 and/or p27 in all cell lines examined . |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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