| Gene name: | HRAS |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HuDE |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//Flow cytometry |
| Description: | Results confirmed that many H-RasV12 expressing cells showed premature aging within one day from the transfection.Microscope analysis of cells transfected with H-RasV12 showed a senescent phenotype within one day from the end of the selection, with a flattened cellular morphology, followed by detachment from the adhesion surface. Besides, H-RasV12 transfected cells showed a cell growth arrest that was already evident one day after the end of the selection, compared to cells transfected with the empty vector alone as control. Interestingly, analysis by flow cytometry showed that significant fractions of H-RasV12 expressing cells are still in G0/G1 and S-phase of the cell cycle, despite their arrested state and altered morphology. Indeed, cell cycle analysis showed that transfected cells had a 14.3% reduction of G0/G1 phase cells and a 6.4% increase of G2/M phase cells, in comparison with control cells. Moreover a 5.2% increase of cells in sub-G0 phase was present in H-RasV12 expressing cells compared to the control, suggesting a possible apoptosis induction. |
| Target gene: | GAPDH |
| Official symbol(s): | GAPDH |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Biochemical assay |
| Target gene description: | We observed a significant depletion of GAPDH activity in H-RasV12 transfected cells compared to the control cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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