| Gene name: | IL6 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HCT116 |
| Experiment: | SA-β-gal activity assay//Western blot//Knockdown |
| Description: | Senescence was induced by either gp130 knockdown, IL-6 neutralization,or IL-6 knockdown.inhibition of the IL-6-dependent activation of the JAK1– STAT3 pathway induced senescence following DNA damage. |
| Regulatory pathway: | JAK1-STAT3 |
| R-AG-Pathway: | Activation |
| Official symbol(s): | JAK1-STAT3 |
| Pathway experiment: | Western blot |
| Pathway description: | To confirm that IL-6 is responsible for the phosphorylation of STAT3 and JAK1, an IL-6 neutralizing antibody (Jo-2) was added to the culture medium 1 day after treatment with doxorubicin.Western blot analysis showed that the Jo-2 antibody blocked the phosphorylation of JAK1 and STAT3 but not of p53 and H2AX.These results suggest that autocrine IL-6 activates the JAK1–STAT3 signaling pathway. |
Annotation:
Loading,please wait...