| Gene name: | TFDP1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HeLa,A549,WI-38,Saos-2 |
| Gene ID: | 7027 |
| Category: | protein coding |
| Phenotype: | Cervical cancer |
| Experimental category: | HL |
| PMID: | 22012588 |
| Experiment: | SA-β-gal activity assay//Knockdown |
| Description: | Based on SA-β-Gal activity after DP1 or DP2 siRNA transfection, HeLa, A549 and WI-38 cells, but not p53-null Saos-2 cells, exhibited an increased percentage of SA-β-Gal-positive cells only upon DP1 depletion. The acute loss o DP2 failed to induce senescence. |
| Regulatory pathway: | P53//P21 |
| R-AG-Pathway: | --//-- |
| Official symbol(s): | TP53//CDKN1A |
| Pathway experiment: | qRT-PCR//Western blot//Knockdown |
| Pathway description: | In all cells examined, the acute loss of DP1, but not DP2, induced a p21Waf1/Cip1 mRNA level comparable to that in the control RNA-treated cells .The accumulation of p53 and p21Waf1/Cip1 protein was clearly detected in DP1-silenced cells, whereas the knock-down of DP2 alone had no effect on the p53 and p21Waf1/Cip1 protein levels in any of the cells that were examined, compared with the control RNA-transfected cells. |
Annotation:
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