| Gene name: | BMP7 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MCF-7 |
| Gene ID: | 655 |
| Category: | protein coding |
| Phenotype: | Breast cancer |
| Experimental category: | L |
| PMID: | 27696331 |
| Experiment: | Cell morphological analysis//Western blot//Telomerase activity assay |
| Description: | In the BMP7 treated cell cultures, we observed the cells characteristics of enlarged and flattened cell morphology, greater cytoplasm/nuclear ratio, and expressions of cell senescence markers such as β-galactosidase and p16 (Janzen et al., 2006;Molofsky et al., 2006).Treatment of MCF-7 cells with BMP7 (30 ng/mL, 15 h in every two-day for two weeks) resulted in a marked increase in the incidence of cell senescence. The increase in cell senescence in the BMP7-treated cultures was associated with reduced cell numbers and protein concentrations (not shown), decreased telomerase activity. The inhibition of telomerase activity in these cells was by 60%–70%.Consistent with cell senescence, BMP7-treated cell cultures showed increased p53, p21 and p16. |
| Target gene: | HTERT |
| Official symbol(s): | HTERT |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Flow cytometry |
| Target gene description: | The extent of BMP7-induced hTERT gene promoter inhibition was similar to that induced by BMP7 in MCF-7 cells.Overexpression of BMP RII receptor similarly blocked BMP7-induced hTERT gene repression in PMC42 breast cancer cells. |
| Regulatory pathway: | SMAD3 |
| R-AG-Pathway: | -- |
| Official symbol(s): | SMAD3 |
| Pathway experiment: | Immunofluorescence//Luciferase reporter assay |
| Pathway description: | Incubation of cultured MCF-7 cells with BMP7 for different periods of time from 10 min to 2 h resulted in Smad3 phospho rylation. Predominant cytoplasmic staining of phospho-Smad3 was observed in non treated MCF-7 cells, whereas predominant nuclear staining BMP7 treatment and in most cells 30 or 60 min after the BMP7 treatment.BMP7 or TGF-βinduced marked increases in the luciferase reporter gene activity that is under the transcriptional control of the Smad3-specific promoter. |
Annotation:
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