| Gene name: | UBTD1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | Tumor tissue |
| Cell name: | HDF,MKN45 |
| Gene ID: | 80019 |
| Category: | protein coding |
| Phenotype: | Gastric cancer |
| Experimental category: | HL |
| PMID: | 25382750 |
| Experiment: | qRT-PCR //Western blot//Flow cytometry//SA-β-gal activity assay//Trypan blue staining//Knockdown |
| Description: | UBTD1 knockdown increased cell proliferation and down-regulated p53 protein. We further analyzed cell cycle and apoptosis by flow cytometry and dead cells by Trypan blue staining, and found that UBTD1 overexpression indeed induces G1-stage arrest, apoptosis and an increase in dead cells. Frozen tumor tissue slices were stained for SA-β-gal marker and a higher proportion of senescent cells were present in the UBTD1 overexpression group (p<0.05). |
| Regulatory pathway: | MDM2-P53 |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | MDM2-TP53 |
| Pathway experiment: | Western blot//qRT-PCR//SA-β-gal activity assay |
| Pathway description: | We treated MKN45 cells with nutlin-3, a known MDM2 antagonist that stabilizes p53, and found that nutlin-3 upregulated p53 and UBTD1 in a dose-dependent manner.We found that exogenous UBTD1 can pull down both endogenous Mdm2 and p53 , suggesting that these three proteins can form a complex and that UBTD1 might regulate p53 via Mdm2. We overexpressed Mdm2 in UBTD1-overexpressing cells, and found that Mdm2 overexpression could reverse UBTD1-mediated up-regulation of p53 expression . |
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