| Gene name: | PAPSS2 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Tumor tissue |
| Cell name: | MCF-7 |
| Gene ID: | 9060 |
| Category: | protein coding |
| Phenotype: | Breast cancer |
| Experimental category: | HL |
| PMID: | 26250908 |
| Experiment: | Cell morphological analysis//Colony formation assay//Flow cytometry//SA-β-gal activity assay |
| Description: | MCF7 human breast cancer cells treated with PAPSS2 Si were fewer in number and showed poor colony-forming ability compared with control siRNA (Con Si)-transfected cells. PAPSS2-depleted cells accumulated in the G1 phase with no increase in the proportion of cells either in the subG1 phase or that were positive for propidium iodide staining. PAPSS2-depleted cells displayed hallmarks of senescent cells such as enlarged and flattened morphology and SA-β-Gal staining.As PAPSS2 was depleted, the cells showed a loss of phosphorylated pRb with changes of cell cycle regulatory protein levels and accumulation of p53 and p21. |
| Regulatory pathway: | FGFR-AKT-P53-P21 |
| R-AG-Pathway: | -- |
| Official symbol(s): | FGFR1-AKT1-TP53-CDKN1A |
| Pathway experiment: | SA-β-gal activity assay//Western blot |
| Pathway description: | PAPSS2-mediated premature senescence, including a loss of phosphorylated pRb, a decrease in cell number, and an increase in SA-β-Gal positivity, was blocked in both p53?/?and p21?/?HCT116 human colon cancer cells.We confirmed that P APSS2 depletion induced augmented FGFR/AKT activation and p53/p21 accumulation in both A549 and HDF cells . The overexpression of a kinase-inActivate (KI) mutant of FGFR1 (FGFR1-KI) attenuated AKT phosphorylation and p53/p21 accumulation in PAPSS2-depleted cells. |
Annotation:
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