| Gene name: | GKN1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | AGS,MKN1,AGSGKN1,MKN1GKN1 |
| Gene ID: | 56287 |
| Category: | protein coding |
| Phenotype: | Gastric cancer |
| Experimental category: | L |
| PMID: | 25344918 |
| Experiment: | SA-β-gal activity assay//qPCR |
| Description: | Average telomere length and telomerase activity decreased significantly in GKN1-transfected AGS and MKN1 cells, compared to those in mock-transfected cells. Furthermore, unlike mock-transfected cells, AGSGKN1 and MKN1GKN1 cells that stably expressed GKN1 demonstrated shortened telomeres and diminished telomerase activity with additional passages.These stable transfectants from passage 5 showed significant shortening of telomeres, as well as decreased telomerase activity and hTERT mRNA expression in a time-dependent manner. The mean percentage of SA-β-gal-positive AGSGKN1 and MKN1GKN1 stable cells increased by 13% and 5.5% at 24 hr and by 24% and 19% 48 hr (P = 0.0134 and P = 0.0247), respectively. |
| Target gene: | C-MYC//HTERT//TRF1 |
| Official symbol(s): | C-MYC//HTERT//TERF1 |
| R-AG-Target gene: | Downregulation//Downregulation//Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | qPCR//Western blot |
| Target gene description: | GKN1 directly bound to the c-myc protein in the immunoprecipitation assay and down-regulated its expression, as well as inhibited its binding activity to TRF1.Interestingly, c-myc mRNA expression was positively correlated with hTERT expression, whereas GKN1 expression was inversely correlated with c-myc and hTERT mRNA expression in 35 gastric cancer tissues. GKN1 protein expression was positively and inversely correlated with TRF1 and c-myc protein expression. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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