| Gene name: | KRT24 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | Skin |
| Cell name: | Primary human epidermal keratinocyte |
| Experiment: | MTS assay//EdU Assay//Flow cytometry//SA-β-gal activity assay |
| Description: | The results indicated that K24 overexpressed keratinocytes showed decreased proliferation rate, as compared with control cells. Furthermore, the actual proliferative capacity of the K24 overexpressed keratinocytes was then determined by EdU assay. The percentage of proliferating cells from the K24 overexpressed cells was 43% after 72 h of incubation, whereas 78% being detected for the control cells.The result showed that the transfection of K24 decreased the DNA synthesis and induced a G1/S growth phase arrest, as evidenced by 84.09% in the experimental group, and 63.94% in the vector control cells. SA-β-galactosidase staining showed a 3-folds more positive blue cells in K24 overexpressed keratinocytes than those in control cells . |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...