| Gene name: | BTK |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | EJp53,HDF |
| Experiment: | Growth curve assay//SA-β-gal activity assay |
| Description: | Inhibition of BTK by chemical or genetic approaches increased cell proliferation in EJp53 induced to senesce. Indeed, the percentage of cells positive for senescence associated (SA)-β-gal, a widely used marker of senescence (32), was significantly lower when BTK was inhibited, and less cells showed the morphological changes typical of senescence. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | Activation |
| Official symbol(s): | TP53 |
| Pathway experiment: | Western blot//RT-PCR |
| Pathway description: | We found that BTK protein levels were elevated after inducing p53 expression in these cells. Moreover, the colon cancer cell line HCT116, which has wild-type p53, also showed a p53-dependent BTK induction after being treated with DNA damaging agents (the oxidant tBH and doxorubicin), both at protein and mRNA levels. We transfected BTK into EJp53 and observed that it elevated the levels of p53 protein induced by tet removal. |
Annotation:
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