| Gene name: | ING1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Hs68 |
| Experiment: | DAPI staining//Flow cytometry//SA-β-gal activity assay |
| Description: | The occurrence of SAHF in senescent cell nuclei (panel B) and highlights the similarity of nuclear phenotype and DNA staining patterns between senescent cells and cells expressing high levels of INGla.Examining the effect of ING1a on cell cycle distribution showed that overexpression of INGla induced cell cycle arrest increased progressively with time despite culturing in complete medium and the percentage of primary Hs68 cells in the G1 phase.Expression of ING1a efficiently induced the expression of SA-β-gal activity.In contrast, cells overexpressing INGla acquired a senescent-like flattened phenotype and a DNA staining pattern similar to that seen in SAHF. |
| Regulatory pathway: | P16-PRB |
| R-AG-Pathway: | Activation |
| Official symbol(s): | CDKN2A-RB1 |
| Pathway experiment: | Western blot |
| Pathway description: | ING1a expression substantially increased both pl6 and pRb levels. |
Annotation:
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