| Gene name: | E2F1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | Embryo |
| Cell name: | MEF |
| Experiment: | BrdU assay//SA-β-gal activity assay//Knockdown |
| Description: | The ability of MEFs to proliferate decreases with cell passage; knocking out E2F1 attenuates this effect. The proliferation of passage 5 WT MEFs was greatly reduced compared with E2F1 KO MEFs, with all cells visualized by Hoechst stained DNA but DNA synthesis was greatly reduced only in WT cells.by passage 5 a significantly greater proportion of WT MEFs were testing positive for senescence compared with E2F1 KO MEFs, indicated by SA-β-gal activity. |
| Target gene: | FOXO3 |
| Official symbol(s): | FOXO3 |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Luciferase reporter assay//Immunostaining |
| Target gene description: | The reporters alone show some activation of the FKRE-luciferase gene, presumably from endogenous FOXO1/3 proteins. This activation is significantly repressed when the E2F1 plasmid is co-transfected.Moreover, the levels of intracellular ROS,as measured by dichlorofluorescein (DCF) levels, whether in the steady state or under oxidative stress, were significantly reduced in E2F1 KO MEFs compared with WT cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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