| Gene name: | SIRT1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Bone and Adipose |
| Cell name: | B-MSC,A-MSC |
| Experiment: | Knockdown//BrdU assay//PI staining//SA-β-gal activity assay |
| Description: | Compared to control, the knockdown of SIRT1 markedly slowed down the growth rate of both types of MSC, as shown by plotting a curve of the cell growth and by the use of a BrdU incorporation assay.In B-MSCs, SIRT1 knockdown significantly decreases the percentage of cells in S phase,while A-MSCs have significantly more cells in the G0/G1 phase and fewer cells in the S phase and G2–M phase after SIRT1 knockdown.However, in cells from later passages, more SA-β-gal+cells were observed in those cultures where SIRT1 had been knocked down. |
| Target gene: | P16 |
| Official symbol(s): | P16 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | However, in late culture passages, p16 but not p21 is accumulated faster in B-MSCs where SIRT1 has been knocked down.Conversely, overexpression of SIRT1, but not its dominantnegative mutant H363Y , efficiently delayed the accumulation of p16. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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