| Gene name: | IGF1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MCF-7,IMR-90 |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//BrdU assay |
| Description: | IGF-1 treatment of IMR90 or MEF cells led to appearance of cells with feature characteristic of premature cellular senescence, including an enlarged flat cell morphology and increased β-Galactosidase (SA-β-Gal) activity.By contrast, cells treated with IGF-1 remained morphologically large and flat, with marginal BrdU incorporation . |
| Regulatory pathway: | SIRT1-P53 |
| R-AG-Pathway: | Activation |
| Official symbol(s): | SIRT1-TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | IGF-1 significantly inhibited SIRT1 deacetylase activity.We confirmed comparable SIRT1 protein input levels by western blot analysis.IGF-1 treatment led to a marked increase in p53 protein levels in serum-starved MCF7, U2-OS, and IMR90 cells.IGF-1 treatment led to a substantial increase in p53 protein half-life, compared with the control. |
Annotation:
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