| Gene name: | FOXO3 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HUC-F2 |
| Experiment: | SA-β-gal activity assay//qRT-PCR |
| Description: | Proliferative potential and senescence-associated β-galactosidase (SA-β-Gal) activity were then monitored. Our results clearly showed that the dominant Activate form of FOXO3a (FOXO3aTM) significantly promoted proliferation and inhibited the onset of replicative senescence in HUC-F2 cells in a manner similar to SIRT1 [6].We detected a significant elongation of telomere length in HUC-F2 cells transduced with FOXO3aTM. |
| Target gene: | C-MYC//HTERT |
| Official symbol(s): | C-MYC//HTERT |
| R-AG-Target gene: | Upregulation//Upregulation |
| Subcategory: | Binding to promoter |
| Target gene experiment: | Luciferase reporter assay//qRT-PCR |
| Target gene description: | We found that FOXO3a increased the promoter activity of c-MYC and the transcription of the c-MYC gene. These results suggest that FOXO3a activates c-MYC expression, which, in turn,results in enhanced quantities of c-MYC at the hTERT promoter,enhanced transcriptional activation of c-MYC and, as a consequence, upregulation of hTERT gene expression. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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