| Gene name: | ATG7 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | Skin |
| Cell name: | keratinocyte |
| Experiment: | Microarray |
| Description: | PQ treatment induced a transcriptional signature of strong cell cycle arrest and DNA damage signaling in the Atg7 deficient cells. |
| Target gene: | P53//P21//CDK1//H2AX |
| Official symbol(s): | P53//P21//CDK1//H2AX |
| R-AG-Target gene: | --//--//--//-- |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//qRT-PCR//Immunostaining |
| Target gene description: | p53 and the downstream mediator p21 were induced by PQ on mRNA and protein level, and the induction was increased in the knockouts on protein level for both proteins.Using qPCR we could verify that the knockout cells showed higher baseline expression of Cdk1.Using WB we could show that this was reflected on protein level, with a stronger Cdk1 signal in untreated KO. We exposed the cells to UVA, which did not cause a significant rise in positive nuclei in WT cells, but did so in KO cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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