| Gene name: | IHH |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | B-MSC |
| Experiment: | SA-β-gal activity assay//RT-PCR//Western blot//Cell morphological analysis//Colony formation assay//PI staining//Flow cytometry |
| Description: | Surprisingly, the count of BMSC cells stained by SA-β-gal stain increased in cells treated with IHH siRNA.Consistently, aging-related genes, p16, p53, SA-β-gal, and mTOR were downregulated after treatment with rIHH.The IHH siRNA transfected BMSC showed more transparency, slight enlargement,and decreased in cell count compared to the negative control.IHH siRNA-transfected BMSC failed to form colonies contrary to the negative control.G0/G1 cell cycle arrest was associated with BMSC of IHH siRNA. |
| Regulatory pathway: | ROS-MTOR-4EBP1 |
| R-AG-Pathway: | Downregulation//-- |
| Official symbol(s): | ROS1-MTOR-EIF4EBP1 |
| Pathway experiment: | Western blot//Flow cytometry//Colony formation assay//Knockdown |
| Pathway description: | We observed down-regulation of P53 and P16 associated with inhibition of mTORand ROS pathways.The cell cycle results showed that inhibition of mTOR and ROS pathways restricted the G0/G1 cell cycle arrest caused by IHH silencing .The colony forming ability of BMSC caused by IHH knockdown was improved after inhibition of mTOR and ROS in the presence of siRNA IHH;As we expected, knockdown of IHH induced 4EBP1 and p70S6K1/2 phosphorylation but rIHH protein treatment down-regulate the phosphorylation process.Our findings presented anti-aging activity for IHH in BMSC through down-regulation of ROS/mTOR pathways. |
Annotation:
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