| Gene name: | ZBTB48 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | pMSC |
| Experiment: | Western blot//SA-β-gal activity assay//qRT-PCR |
| Description: | Overexpression of TZAP in P2 pMSCs resulted in an increase in the percentage of SA-β-gal-positive cells by 17% (from 6 to 23%). Western blot analysis showed that compared to the control vector, TZAP over-expression in pMSCs significantly increased the protein levels of P21 and P16. Consistent with this finding, qRT-PCR also revealed upregulated transcript levels of p21 and p16Ink4a. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | Upregulation |
| Official symbol(s): | TP53 |
| Pathway experiment: | Western blot |
| Pathway description: | After overexpression of TZAP in pMSCs, western blot analysis showed that overexpression of TZAP led to increased levels of ARF, P53 and P21 but decreased levels of MDM2 compared to those in pMSCs transduced with the control vector. In contrast, knockout of TZAP in pMSCs decreased ARF, P53, and P21 levels but increased MDM2 levels . |
Annotation:
Loading,please wait...