| Gene name: | MIF |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MSC |
| Gene ID: | 4282 |
| Category: | protein coding |
| Phenotype: | Myocardial infarction |
| Experimental category: | L |
| PMID: | 31881006 |
| Experiment: | Knockdown//Western blot//SA-β-gal activity assay |
| Description: | MIF-siRNA treatment of young MSCs significantly downregulated MIF expression, while it significantly elevated p53 and p21 expression. Furthermore, MIF-siRNA treatment remarkably increased the number of SA-β-gal-positive cells among young MSCs.While the overexpression of MIF in aged MSCs enhanced MIF expression, it reduced p53 and p21 expression. Moreover, the percentage of SA-β-gal-positive cells was greatly reduced in MIF-aged MSCs compared with aged MSCs. |
| Target gene: | BECLIN1//LC3-I/II//P62 |
| Official symbol(s): | BECLIN1//MAP1LC3A//P62 |
| R-AG-Target gene: | Upregulation//Upregulation//Downregulation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot |
| Target gene description: | Autophagy has recently been found to inhibit cellular senescence.Overexpression of MIF in aged MSCs significantly induced autophagy, as manifested by the elevated expression of Beclin1 and LC3I/II and the reduced expression of p62. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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