| Gene name: | TRPM7 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | BxPC-3,PANC-1 |
| Gene ID: | 54822 |
| Category: | protein coding |
| Phenotype: | Pancreatic cancer |
| Experimental category: | L |
| PMID: | 22166235 |
| Experiment: | SA-β-gal activity assay//Cell morphological analysis//qRT-PCR//Knockdown |
| Description: | Consistent with the observations in the micrographs with phase contrast, bright field examination reveals that both BxPC-3 and PANC-1 cells with targeted knock down of TRPM7 expression exhibit morphological features indicative of a senescent phenotype. In the BxPC-3 and PANC-1 cells transfected with anti-TRPM7 siRNA but not in the controls, SA β-gal activity was detected.In TRPM7-deficient PANC-1 cells, the mRNA levels of p16INK4A and WRN were elevated whereas that of p27CDKN1B remained unaltered. Similarly, in TRPM7-deficient BxPC-3 cells, the mRNA levels of p16INK4A and WRN were elevated by 1.4-fold and 26%, respectively. |
| Target gene: | WRN |
| Official symbol(s): | WRN |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR |
| Target gene description: | In TRPM7-deficient PANC-1 cells, the mRNA levels of p16INK4A and WRN were elevated whereas that of p27CDKN1B remained unaltered. Similarly, in TRPM7-deficient BxPC-3 cells, the mRNA levels of p16INK4A and WRN were elevated by 1.4-fold and 26%, respectively. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...