| Gene name: | CHEK2 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | Colon |
| Cell name: | BJ,Fibroblast,iG4 Terc?/?Fibroblast |
| Gene ID: | 11200 |
| Category: | protein coding |
| Phenotype: | Telomere dysfunction |
| Experimental category: | L |
| PMID: | 20577265 |
| Experiment: | Knockdown//Western blot//Immunostaining |
| Description: | Infection of late‐passage, primary human BJ fibroblasts with lentiviral vectors expressing a CHK2‐directed shRNA confirmed that CHK2 knockdown abrogated the induction of senescence, resulting in positive selection of CHK2‐knockdown cells and improved proliferation rates of human fibroblast cultures at late passage. Similar results were obtained for mouse ear fibroblasts. Genetic deletion of CHK2 rescued proliferation of iG4 Terc?/? fibroblasts. Immunofluorescence staining of phosphorylated CHK1 showed nuclear staining of phospho-CHK1 in intestinal basal crypts and progenitor cells of iG4 mice. Immunofluorescence on CHK1-shRNA-treated cells confirmed the staining specificity. In addition, western blot analysis of intestinal epithelium reconfirmed the activation of CHK1 in iG4 and iG4 Chk2?/? mice. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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