| Gene name: | MYC |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | hTERT-immortalized WRN?/? Fibroblast strains AG00780,hTERT-immortalized WRN?/? Fibroblast strains AG03141 |
| Experiment: | SA-β-gal activity assay//Microarray//Knockdown |
| Description: | However, within 2–3 passages, ～30%–70% of the WRN–/– cells expressed senescence-associated (SA-) β-galactosidase and lost proliferative capacity. The senescent phenotype in c-myc-transduced WRN–/– cells was also confirmed at the gene expression level by microarray analysis, which demonstrated elevated expression of several genes characteristic of replicative senescence, such as the matrix proteases. In contrast, only a small percentage of the hTERT+ WRN–/– cells transduced with a control retroviral vector expressed SA-β-gal (～1%), similarly to hTERT-immortalized normal fibroblasts (hTERT+, from two independent donors) upon MYC overexpression. |
| Target gene: | WRN |
| Official symbol(s): | WRN |
| R-AG-Target gene: | Upregulation |
| Subcategory: | Unclear |
| Target gene experiment: | CHIP//qRT-PCR//Co-IP |
| Target gene description: | Comparable results demonstrating increased WRN A and B site binding by MYC were obtained in four different cell lines expressing deregulated c-myc.In vivo binding of MYC to the WRN promoter and a modest but consistent elevation in histone H4 acetylation, as observed for other MYC-target genes (Frank et al. 2001), was also shown by duplex PCR in the B-cell line P-493-6 that expresses a Tet-Myc-repressible gene. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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