| Gene name: | HNRNPA1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HUVEC,A549 |
| Experiment: | CCK-8 assay//SA-β-gal activity assay//Knockdown//Flow cytometry//qRT-PCR//Western blot |
| Description: | HNRNPA1-KD led to a higher percentage of positive SA-β-Gal stained cells and slower cell growth rate in both cell types. Besides, HNRNPA1-KD HUVECs and A549 cells also showed arrested G2/M phase, decreased LMNB1 expression, increased ROS production, and altered expression of senescence-associated molecular markers (including CDK1, CDK2, CCNB1, and IL6), resembling to the effects caused by HN1-KD. |
| Target gene: | HN1-L |
| Official symbol(s): | JPT2 |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR//Knockdown |
| Target gene description: | Knockdown of HNRNPA1 or HNRNPM could promote the relative expression of HN1-L compared to total expression (HN1-L and HN1-S, labelled as HN1-T), but HNRNPA1 demonstrated the maximun effect, so HNRNPA1 was choosed for further investigation. the increased L/T ratio in HNRNPA1-KD cells could be reversed with HNRNPA1 overexpression. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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