| Gene name: | CEBPB |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | PB-TRE,PB-CEBPB,LNCaP-shNTV |
| Gene ID: | 1051 |
| Category: | protein coding |
| Phenotype: | Prostate cancer |
| Experimental category: | HL |
| PMID: | 25772238 |
| Experiment: | SA-β-gal activity assay//Flow cytometry//qRT-PCR//Immunostaining |
| Description: | Cells expressing shCEBPB or shNTV both went into G1 cell cycle arrest when cultured in androgen depleted media (ADM).Compared to control PB-TRE, PB-CEBPB cells had a significantly increase in the number SA-β-gal positive cells and the level of cell granularity, as assessed by side scatter.overexpression of C/EBPβ led to a significant increase in the transcript levels of two SASP-associated genes, IL8 and IGFBP3. When cultured in ADM, LNCaP-shNTV cells display a 2-fold increase in the number of HF positive cells.the proportion of Ki67-negative cells after one week of culture in ADM was 2-fold lower in cells lacking C/EBPβ. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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