| Gene name: | RAPGEF4 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | ESC |
| Gene ID: | 11069 |
| Category: | protein coding |
| Phenotype: | Decidualization of Human Endometrial Stromal Cell |
| Experimental category: | L |
| PMID: | 24169561 |
| Experiment: | SA-β-gal activity assay//Western blotR//Knockdown//Cell morphological analysis |
| Description: | The intensity of SA-β-Gal staining was significantly increased in the EPAC2 siRNA-treated group compared with the control.When the expression of p53 and p21 was examined in these cells, knockdown of EPAC2 suppressed p53 levels and increased p21 levels.Double knock-down of EPAC2 and p21 reduced the ratio of SA-β-Gal-positive cells when compared with single knock-down of EPAC2. |
| Target gene: | CRT |
| Official symbol(s): | CRT |
| R-AG-Target gene: | Inhibition |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//Knockdown//qRT-PCR |
| Target gene description: | Consistent with the results of LC-MS/MS analysis, EPAC2 knock-down significantly suppressed CRT expression, regardless of the presence of cAMP analogs.real-time RT-PCR analysis showed that EPAC2 knock-down inhibited expression of CRT mRNA in each group, with or without cAMP analogs. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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