| Gene name: | SERPINE1 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HUVEC |
| Experiment: | SA-β-gal activity assay |
| Description: | CPAI-Q123K, HPAI-RR, and the negative control PAI-L all failed to directly induce HUVEC senescence, whereas CPAI resulted in SA-β-gal-positive staining in nearly 40% of HUVECs. Moreover, treatment of senescent HUVECs with the PAI-1 inhibitor PAI-039 significantly decreased the senescent cell ratio in a dose-dependent manner.we added PAI-039 into young HUVECs infected with Ad-U6 or Ad-SIRT1 RNAi and observed that PAI-1 inhibition significantly reversed the senescence induced by SIRT1 knockdown in HUVECs. The results showed that treatment with an exogenous stable form of PAI-1 CPAI, but not CPAI-Q123K, HPAI-RR, and PAI-L, nearly completely blocked the antisenescence effect of adenovirus-mediated SIRT1 overexpression in senescent HUVECs. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...