| Gene name: | ICMT |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | Fibroblast |
| Gene ID: | 23463 |
| Category: | protein coding |
| Phenotype: | Hutchinson-Gilford progeria syndrome |
| Experimental category: | L |
| PMID: | 23686339 |
| Experiment: | Knockdown//Growth curve assay |
| Description: | As expected,Zmpste24?/? Icmt+/+ fibroblasts proliferated slowly and senesced prematurely. In contrast, Zmpste24?/? Icmthm/hm and Zmpste24?/? Icmt△/△ cells proliferated at rates similar to those of wildtype cells. |
| Target gene: | PRELAMIN A |
| Official symbol(s): | PRELAMIN A |
| R-AG-Target gene: | Inhibition |
| Subcategory: | Unclear |
| Target gene experiment: | DAPI |
| Target gene description: | prelamin A was mainly found at the nuclear rim in Zmpste24?/?Icmt+/+hepatocytes,colocalizing with LAP2b.In contrast,prelamin A in Zmpste24?/?Icmthm/hm hepatocytes was abundant in the nucleoplasm. |
| Regulatory pathway: | AKT-MTOR |
| R-AG-Pathway: | -- |
| Official symbol(s): | AKT1-MTOR |
| Pathway experiment: | Knockdown//Western blot |
| Pathway description: | We observed higher levels of phosphorylated AKT and greater mTOR activation in Zmpste24?/? Icmthm/hm cell lysates than in Zmpste24?/? Icmt+/+ lysates, evident by increased phosphorylation of its downstream targets ribosomal protein S6 and 4E-BP1. Similar results were observed in comparisons of Zmpste24+/+Icmthm/hm and Zmpste24+/+ Icmt+/+ cells. Consistent with increased rates of proliferation, Zmpste24?/? Icmthm/hm cells had lower levels of the cyclin-dependent kinase inhibitors p27KIP1 and p21CIP1 and the tumor suppressors p16INK4A and phosphorylated retinoblastoma protein (Rb). |
Annotation:
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