| Gene name: | KDM4A |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | IMR-90 |
| Experiment: | SA-β-gal activity assay//Knockdown |
| Description: | Compared to cells transfected with siGFP, cells depleted of JMJD2A exhibited a flattened vacuolated morphology accompanied by a positive staining to the senescence-associated β-galactosidase assay(SA-β-gal).However, the knockdown of JMJD2A led to the accumulation of PML nuclear bodies,another well- characterized senescence marker. |
| Target gene: | CHD5 |
| Official symbol(s): | CHD5 |
| R-AG-Target gene: | Inhibition |
| Subcategory: | Unclear |
| Target gene experiment: | CHIP//qRT-PCR//Knockdown |
| Target gene description: | Among the two putative JMJD2A-binding sites identified by ChIP-on-chip on the CHD5 promoter, only the site located at +741 was enriched by ChIP-qPCR.JMJD2A depletion also led to a concomitant increase of CHD5 mRNA and protein.We detected an increase of H3K9(me3) levels at the CHD5 promoter after depletion of JMJD2A . |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | Downregulation |
| Official symbol(s): | TP53 |
| Pathway experiment: | SA-β-gal activity assay//Knockdown |
| Pathway description: | Transfection of siRNAs targeting JMJD2A triggered senescence in p16-depleted cells but not in p53-depleted cells, suggesting that the senescence response triggered by decreased JMJD2A levels relies primarily on the p53 pathway. Ectopic expression of JMJD2A caused a significant decrease of both p53 and p21 in Ras-expressing cells. |
Annotation:
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