| Gene name: | SIRT1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | BM-MSC |
| Gene ID: | 23411 |
| Category: | protein coding |
| Phenotype: | Osteoporosis |
| Experimental category: | HL |
| PMID: | 30690778 |
| Experiment: | BrdU assay//Colony formation assay//SA-β-gal activity assay//Western blot |
| Description: | Compared with cells from the vehicle-treated group, resveratrol-treated cells had a higher percentage of BrdU positive cells and numbers of ALP+ CFU-f colonies. Resveratrol-treated M-MSCs from WT mice had similar numbers of ALP+ CFU-f colonies as vehicle treated M-MSCs from Sirt1TG mice, and resveratrol-treated M-MSCs from Sirt1TG mice had the highest numbers of ALP+ CFU-f colonies.Consistent with these results, p16 expression levels and senescence associated β-Gal positive areas were increased in H-BM-MSCs from old people and decreased in response to resveratrol, and these changes were blocked by the Sirt1-specific knockdown virus. |
| Target gene: | BMI1 |
| Official symbol(s): | BMI1 |
| R-AG-Target gene: | Activation |
| Subcategory: | Unclear |
| Target gene experiment: | Western blot//IP |
| Target gene description: | The results showed that Sirt1 could bind to Bmi1.Immunoprecipitation with an anti-Bmi1 antibody showed a dose-dependent upregulation of Sirt1 protein expression associated with decreased expression of acetylated lysine. Western blot data from nuclear lysates demonstrated that the dose-dependent upregulation of Sirt1 was concomitant with the upregulation of Bmi1 expression. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...