Aging Overview

Aging gene

Gene name: GNMT
Aging type: Accelerate
Aging characteristic:
Tissue type: --
Cell name: HuH-7-GNMT,HuH-7-GFP,HepG2
Gene ID: 27232
Category: protein coding
Phenotype: Hepatocellular carcinoma
Experimental category: L
PMID: 22160218
Experiment: SA-β-gal activity assay//Flow cytometry//MTT assay
Description: The growth rate of HuH-7-GNMT cells was significantly slower than that of HuH-7-GFP cells. Similar results were also observed in HepG2 cells overexpressing GNMT. Cell cycle analysis showed that, 36 h after the cells entered the cell cycle, the percentages of cells in the G2/M phase for HuH-7-GNMT cells and HuH-7-GFP cells were 23.8% and 10.4%, respectively.Furthermore, SA-β-gal assay demonstrated that HuH-7-GNMT cells had asignificantly higher rate of positive staining than HuH-7-GFP cells.


Regulatory relationship

Target gene: DEPTOR
Official symbol(s): DEPTOR
R-AG-Target gene: --
Subcategory: Unclear
Target gene experiment: FRET-AB assay//Co-IP
Target gene description: Immunoprecipitation of either HAtagged DEPTOR or endogenous DEPTOR coprecipitated FLAG-tagged GNMT. In addition, we detected endogenous DEPTOR in GNMT immunoprecipitants prepared from mouse liver. The results showed that the FRET efficiency between full-length GNMT and DEP domains of DEPTOR decreased significantly. In addition, a >50% decrease of the FRET efficiency was found between full-length DEPTOR and the N-terminal of GNMT.

Regulatory pathway: MTOR-RAPTOR
R-AG-Pathway: Activation
Official symbol(s): MTOR-RPTOR
Pathway experiment: Western blot
Pathway description: The results showed that overexpression of GNMT led to increases of both 4E-BP phosphorylation and cell size. In addition, overexpression of DEPTOR in HuH-7-GNMT stable cells resulted in the neutralization of the effect of GNMT on 4E-BP phosphorylation.


Aging network

Annotation:

The green line represents Upregulation.

The purple line represents Downregulation.

The orange line represents Activation.

The yellow line represents Inhibition.

The gray line represents Unclear.



Pathway view

About risk SNP and eQTL

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