| Gene name: | PIP4K2B |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | BT-474 |
| Gene ID: | 8396 |
| Category: | protein coding |
| Phenotype: | Breast cancer |
| Experimental category: | HL |
| PMID: | 24209622 |
| Experiment: | SA-β-gal activity assay//Knockdown//Immunostaining |
| Description: | We observed a significant increase in reActivate oxygen species (ROS), in oxygen consumption, and in β-galactosidase staining in the BT474 cells in response to knockdown of both PI5P4Kα and β consistent with ROS-induced senescence.Decrease in cell proliferation in response to knockdown of both PI5P4Kα and β. |
| Regulatory pathway: | PI3K-AKT |
| R-AG-Pathway: | -- |
| Official symbol(s): | PIK3CA-AKT1 |
| Pathway experiment: | Western blot//Knockdown |
| Pathway description: | This increase in AKT phosphorylation could be explained by an increase in PI-3,4,5-P3 levels in response to knockdown of both PI5P4Ka and b. Surprisingly, we observed a dramatic decrease in PI-3,4-P2 in the double-knockdown cells.The levels of INPP4B were virtually undetectable in BT474 cells prior to the knockdown of PI5P4Ka and b but were quite high following the knockdown, explaining the drop in PI-3,4-P2 levels . |
Annotation:
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