| Gene name: | FANCD2 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | Skin |
| Cell name: | Fancd2+/+ epithelial cell |
| Gene ID: | 2177 |
| Category: | protein coding |
| Phenotype: | Fanconi anemia |
| Experimental category: | HL |
| PMID: | 23806336 |
| Experiment: | SA-β-gal activity assay//Knockdown |
| Description: | To test this hypothesis, we examined the level of senescence-associated (SA)-β-galactosidase staining, a measure of in vivo senescence (Lin et al., 2010), in skin extracted from the TPA-exposed animals. Consistent with the reduced proliferation observed in TPA-treated Fancd2+/+ epithelial cells, we detected a higher accumulation of SA-β-galactosidase staining in this tissue compared to Fancd2+/-epithelium. |
| Target gene: | TAP63//USP1 |
| Official symbol(s): | TAP63//USP1 |
| R-AG-Target gene: | Upregulation//-- |
| Subcategory: | Unclear |
| Target gene experiment: | CHIP//Western blot |
| Target gene description: | Consequently, we performed chromatin immunoprecipitation (ChIP) assays to determine whether FANCD2-Ub could directly bind to the promoter region of TAp63. The promoter regions of the p63 gene have previously been analyzed (Buckley et al., 2011; Waltermann et al., 2003). We initially divided the promoter of TAp63 into six regions(A1–A6)The nuAs predicted, A549 cells expressing USP1 shRNA showed an increase in the level of FANCD2-Ub. The reduction of USP1 expression by shRNA, and the increased FANCD2-Ub expression, inhibited the colony formation of A549 cells in soft agar and inhibited the tumor xenograft growth in nude mice.The ChIP results demonstrate that FANCD2-Ub can bind to relevant cisacting regulatory sequences of the TAp63 gene, leading to enhanced TAp63 expression and enhanced cellular senescence. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
Loading,please wait...