| Gene name: | TERT |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | U937 |
| Gene ID: | 7015 |
| Category: | protein coding |
| Phenotype: | Atherosclerosis |
| Experimental category: | L |
| PMID: | 21106948 |
| Experiment: | SA-β-gal activity assay//Knockdown//qRT-PCR |
| Description: | MPM isolated from first-generation TERT-deficient mice significantly upregulated senescence associated -galactosidase activity, an established biomarker of replicative senescence. Furthermore, TERT-deficient macrophages revealed increased transcript levels of several genes causally involved in replicative senescence,26 including p16,p21, and the retinoblastoma protein. |
| Regulatory pathway: | NF-κB |
| R-AG-Pathway: | -- |
| Official symbol(s): | NFKB1 |
| Pathway experiment: | CHIP |
| Pathway description: | We next performed chromatin immunoprecipitation assays using primer pairs that cover the NF-KB site at 592/580 in the human TERT promoter to determine whether NF-KB subunits are recruited to the endogenous TERT promoter. These assays confirmed that LPS, oxLDL, and TNF- induce the recruitment of both NF-KB subunits to the consensus site in the human TERT promoter. Similarly, chromatin immunoprecipitation analysis in primary MPM revealed that oxLDL induces a strong recruitment of p65 and p50 to a region encompassing an NF-KB site at 250/238 in the murine TERT promoter. |
Annotation:
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