| Gene name: | TOP1 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HCT116 |
| Experiment: | SA-β-gal activity assay//Knockdown//Flow cytometry//Clonogenic assay |
| Description: | We observed using clonogenic assays that sn38 induced a complete inhibition of cell proliferation. Using beta-galactosidase staining, we also noticed an induction of senescence following genotoxic treatment.control cells were synchronized in the G2 phase of the cell cycle after 48-72 hr. |
| Target gene: | AURORA-A |
| Official symbol(s): | AURORA-A |
| R-AG-Target gene: | -- |
| Subcategory: | Unclear |
| Target gene experiment: | qRT-PCR//Western blot//Knockdown |
| Target gene description: | Although this was the cell cycle stage when Aurora-A expression was supposed to be maximal, results indicated that the expression of the kinase was downregulated in response to sn38, both at the protein and mRNA levels.Finally, these experiments have also been repeated in a different colorectal cell line and sn38 also downregulated Aurora-A in HT29 cells. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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