| Gene name: | FBXO31 |
| Aging type: | Accelerate |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MCF-7 |
| Experiment: | SA-β-gal activity assay//BrdU assay |
| Description: | we found that ectopic expression of FBXO31 in MCF7 cells promoted growth arrest, as evidenced by reduced proliferation and decreased DNA replication (BrdU incorporation), and induced senescence, as evidenced by positive staining for senescence-associated β-gal. |
| Target gene: | MDM2//P53//ATM |
| Official symbol(s): | MDM2//P53//ATM |
| R-AG-Target gene: | Downregulation//Upregulation//-- |
| Subcategory: | Phosphorylation |
| Target gene experiment: | Western blot//Co-IP |
| Target gene description: | The immunoblot shows that ectopic expression of FBXO31 resulted in decreased levels of MDM2, which, as expected, were accompanied by increased levels of p53 and p21.The presence of MDM2 in the FBXO31 immunoprecipitate, which was increased by lactacystin addition. The reciprocal coimmunoprecipitation experiment showed the presence of myc-FBXO31 in the MDM2 immunoprecipitate.FBXO31 failed to reduce MDM2-6A levels, confirming the essential role of ATM in FBXO31-directed degradation of MDM2 |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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