| Gene name: | ACKR3 |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | C4-2B |
| Gene ID: | 57007 |
| Category: | protein coding |
| Phenotype: | Prostate cancer |
| Experimental category: | HL |
| PMID: | 28596572 |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//Knockdown |
| Description: | After one month in culture, no further proliferation could be observed, and all cells had developed a broad, flattened morphology with numerous long protrusions.In proliferation-arrested CRISPR-Cas9 mutated C4-2B colonies, strong blue staining was observed in nearly all cells indicating cellular senescence. |
| Target gene: | ANDROGEN RECEPTOR(AR) |
| Official symbol(s): | ANDROGEN RECEPTOR(AR) |
| R-AG-Target gene: | Downregulation |
| Subcategory: | Binding to promoter |
| Target gene experiment: | CHIP//Luciferase reporter assay |
| Target gene description: | Chromatin immunoprecipitation (ChIP) assays were performed on the genomic CXCR7 promoter in LNCaP cells in the presence and absence of androgen. The promoter of prostate specific antigen (PSA) was used as a positive control for AR binding and showed a significant enrichment with androgen stimulation compared to androgen deprived cells. In the CXCR7 promoter, there was a significant increase in AR binding at the predicted ARE 2 region (R1881: 2.35±0.54 vs. CDFBS: 0.49±0.09) as well as the region containing the closely spaced (7 nt separation) AREs 4 and 5 (ARE 4/5) (R1881: 1.65±0.14 vs. CDFBS: 1.11±0.12). Androgen stimulation also increased AR enrichment of the ARE 1 and ARE 3 regions, but did not reach significance. There was no change in enrichment at the ARE 6 region. |
| Regulatory pathway: | EGFR |
| R-AG-Pathway: | -- |
| Official symbol(s): | EGFR |
| Pathway experiment: | Western blot//Histochemical staining |
| Pathway description: | We observed substantially reduced CXCR7-ARRB1 and increased CXCR7-ARRB2 interactions in the CXCR7-mutant LNCaP cell line compared to WT CXCR7 LNCaP cells. These data reveal that the lost protein region containing amino acids 114 to 243 altered protein-protein interactions necessary for the observed ARRB-EGFR-CXCR7 regulatory axis, providing a potential explanation for disrupted EGF-mediated mitogenic signaling. |
Annotation:
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