| Gene name: | MSN |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | HDMEC |
| Experiment: | Cell morphological analysis//SA-β-gal activity assay//Knockdown//Western blot//RT-PCR |
| Description: | Moesin knock-down HDMECs showed the characteristics of aging, i.e., they were large, flat, and stellate, beginning at the early stage of passage 6. Moesin knock-down HDMECs from the early stage of passage 6 were stained blue with SA-β-gal. HDMECs at late passages higher than passage 25 stained blue in all serial subcultures. In comparison with control vector-treated HDMECs, the doubling time of moesin knock-down HDMECs was delayed by 3 h at passage 6 and 10 h at passage 22. The doubling level of the cumulative cell population in moesin-knockdown HDMECs was much less than that in virus-untreated HDMECs. Moesin knock-down HDMECs expressed less moesin RNA than control vectortreated HDMECs. In addition, the RNA expression of p16 in HDMECs at late passages and moesin knockdown HDMECs was higher than that in control vectortreated HDMECs.In applying Western blotting, reduced expression of moesin and higher expression of p16 were observed in moesin knock-down HDMECs, compared to control vectortreated HDMECs. |
| Regulatory pathway: | -- |
| R-AG-Pathway: | -- |
| Pathway experiment: | -- |
| Pathway description: | -- |
Annotation:
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