Gene name: | BTK |
Aging type: | Accelerate |
Aging characteristic: |
Tissue type: | Brain |
Cell name: | -- |
Experiment: | SA-β-gal activity assay//Knockdown//qRT-PCR |
Description: | To test whether BTK inhibition could prevent the age-dependent buildup of senescent cells in the brain, we measured the expression of different markers of senescence in brains of control and ibrutinib-treated Zmpste24?/? mice.This was concomitant with a decrease in p53 and p16 levels, as expected. In line with this, mRNA levels of BTK (which is a transcriptional target of p53 (Althubiti et al., 2016)) and other markers of senescence were also decreased in the brain samples. p53 mRNA levels did not change significantly, which is compatible with the post-translational effects of BTK on p53 levels (Althubitiet al., 2016). The reduction in senescent cell accumulation in brains of treated mice was confirmed by a whole organ SA-β-gal staining. |
Annotation:
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