| Gene name: | SBDS |
| Aging type: | Prevent |
| Aging characteristic: |
| Tissue type: | -- |
| Cell name: | MRC-5 |
| Experiment: | SA-β-gal activity assay//Knockdown//BrdU assay//Western blot |
| Description: | Next, we used siRNA SMARTpools to EFL1 and LSG1 and observed the expected reduction in BrdU incorporation, induction of acidic β‐galactosidase activity and induction of p16 immunoreactivity. |
| Regulatory pathway: | P53 |
| R-AG-Pathway: | -- |
| Official symbol(s): | TP53 |
| Pathway experiment: | Knockdown//BrdU assay//qRT-PCR//DAPI staining |
| Pathway description: | E6（E6 abrogated p53 expression） expression led to maintained BrdU incorporation upon LSG1 knockdown, indicating p53 dependence. Expression of E7（E7 expression enhanced p53 levels）did not rescue the inhibition of cell cycle elicited by LSG1 knockdown. Once again, inhibition of the p53 pathway led to bypass of shLSG1‐induced proliferative arrest . Finally, we used shRNA to p53 to follow the growth characteristics of cell lines transduced with shRNA to LSG1. p53 knockdown resulted in greatly accelerated growth rates in vector control cells, and the knockdown of LSG1 failed to inhibit growth in these cells. |
Annotation:
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